Matches in Nanopublications for { <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> ?p ?o ?g. }
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- step description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." assertion.
- step description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." assertion.
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- step reagentUsed CHEBI_41609 assertion.
- step reagentUsed CHEBI_18153 assertion.
- step reagentUsed CHEBI_35225 assertion.
- step reagentUsed CHEBI_29362 assertion.
- step labEquipmentUsed CHEBI_35225 assertion.
- step bioSampleUsed CHEBI_41609 assertion.
- step bioSampleUsed CHEBI_18153 assertion.
- step bioSampleUsed CHEBI_35225 assertion.
- step bioSampleUsed CHEBI_29362 assertion.
- step bioSampleUsed "microscopy" assertion.
- step hasExperimentalInput CHEBI_29362 assertion.