Matches in Nanopublications for { <http://purl.org/np/RAVJhdaFi9jN_l4fYtseN0muLo_4nwFBylO6M8DrdckmU#step> ?p ?o ?g. }
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- step type Step assertion.
- step description "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." assertion.
- step reagentUsed CHEBI_8984 assertion.
- step reagentUsed CHEBI_29234 assertion.
- step reagentUsed CHEBI_17992 assertion.
- step reagentUsed _4 assertion.
- step reagentUsed _6 assertion.
- step reagentUsed _5 assertion.
- step labEquipmentUsed BAO_0010014 assertion.
- step labEquipmentUsed _3 assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalInput _1 assertion.