Matches in Nanopublications for { <http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo#step> ?p ?o ?g. }
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- step type Step assertion.
- step description "Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield." assertion.
- step isStepOfPlan plan assertion.
- step precedes step assertion.
- step reagentUsed _2 assertion.
- step labEquipmentUsed OBI_0400155 assertion.
- step bioSampleUsed _1 assertion.