Matches in Nanopublications for { ?s <http://purl.org/dc/elements/1.1/description> ?o ?g. }
- step description "Add an equal volume of isopropanol to tube. Mix contents gently and keep undisturbed for 5-10 min. The white thread-like structure of precipitated DNA will be seen in suspension (Figure 1)" assertion.
- step description "Aspirate liquid without disturbing pellet. Keep tube open to air dry pellet. Do not over dry pellet, as over dried DNA will be difficult to dissolve" assertion.
- step description "Dissolve pellet in molecular biology grade water and analyze DNA by agarose gel electrophoresis and spectrophotometer for its purity and integrity" assertion.
- step description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.
- step description "The cells in interphase need to be aspirated without delay. If the tubes are kept standing for more than 10 min, PBMCs from the interphase will get disturbed and start settling down." assertion.
- step description "Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium." assertion.
- step description "Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase." assertion.
- step description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers." assertion.
- step description "Take 4 ml of Ficoll Histopaque in a 15 ml centrifuge tube." assertion.
- step description "Collect 4 ml of human venous blood sample in heparinised vials (BD biosciences) and mix well by gently inverting the tube several times." assertion.
- step description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.
- step description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.
- step description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers." assertion.
- RAl6W1vijjCZIHiKNUk1F_bq1uLVCpYhAb6wsvU8NEHVk.step description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." RAl6W1vijjCZIHiKNUk1F_bq1uLVCpYhAb6wsvU8NEHVk.assertion.
- step description "Decontaminate the tools for muscle dissection, including forceps, scalpels, and scissors, with RNaseZAPTM wipes and rinse thoroughly with double-distilled water (ddH2O). Decontaminate the procedure area by spraying with 70% ethanol." assertion.
- RAkLJW7vIsnKKJDf1iswdgtFPQSo3lEG_z8DhHfD7dofE description "This set includes the nanopublications of the submissions, auxiliary class definitions, reviews, responses, and decisions for the special issue at the journal Data Science by IOS Press." pubinfo.
- RAaZp4akBZI6FuRzIpeksyYxTArOtxqmhuv9on-YssEzA description "A dataset of 327 off-label drug indications found in PubMed articles. With additional information on the context of the indications, such as the target group age range (adult/children), or if the target group has a specific phenotype. Drugs are identified by their DrugBank IDs, and conditions are identified by their MONDO, EFO, or HPO IDs. Curated by Ricardo de Miranda Azevedo. See https://github.com/MaastrichtU-IDS/off-label-drug-indications-dataset for more details." pubInfo.
- RAVQGOE6nCeX6Tp1rqBOKleQElWhgb1PGHvbIvH1TCE1A description "A dataset of 1971 drug indications retrieved from the PREDICT publication and used as reference dataset for the OpenPredict model. See https://github.com/MaastrichtU-IDS/translator-openpredict for more details." pubInfo.
- RA556Vv9lmc7jqKZkRV_5CP8Xz4GZaVbgUdIsye7tynN0 description "A dataset of 1971 drug indications retrieved from the PREDICT publication and used as reference dataset for the OpenPredict model. See https://github.com/MaastrichtU-IDS/translator-openpredict for more details." pubInfo.
- RAWWaT9M_Nd8cVm_-amJErz60Ak__tkS6ROi2P-swdmMw description "A dataset of 1971 drug indications retrieved from the PREDICT publication and used as reference dataset for the OpenPredict model. See https://github.com/MaastrichtU-IDS/translator-openpredict for more details." pubInfo.