Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAysi2SPEKm8TaYscHjRtWBr9tT0a2IA9d0jhRLRDP8bc#assertion>. }
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- step type Step assertion.
- _1 label "recalcitrant samples" assertion.
- _3 label "pestle" assertion.
- _2 label "recalcitrant samples" assertion.
- _5 label "extraction buffer" assertion.
- _4 label "mortar" assertion.
- CHEBI_17997 label "liquid nitrogen" assertion.
- EFO_0000992 label "shoot" assertion.
- PO_0025034 label "leaf" assertion.
- PO_0009005 label "root" assertion.
- step description "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples." assertion.
- step reagentUsed _5 assertion.
- step reagentUsed CHEBI_17997 assertion.
- step labEquipmentUsed _3 assertion.
- step labEquipmentUsed _4 assertion.
- step bioSampleUsed _2 assertion.
- step bioSampleUsed EFO_0000992 assertion.
- step bioSampleUsed PO_0025034 assertion.
- step bioSampleUsed PO_0009005 assertion.
- step hasExperimentalInput _1 assertion.
- step hasExperimentalInput EFO_0000992 assertion.
- step hasExperimentalInput PO_0025034 assertion.
- step hasExperimentalInput PO_0009005 assertion.