Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RA_YF4ofhZI0leT3rK8aCWbOyf9byQvyueEVBnQIhSM0M#assertion>. }
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- step type Step assertion.
- _2 label "10% SDS" assertion.
- OBI_0400155 label "water bath" assertion.
- _1 label "10 mg/ml Proteinase K" assertion.
- step description "Add 1 ml of 10% SDS solution (final concentration, ~1%) and 20 µl of 10 mg/ml Proteinase K solution (final concentration 20 µg/ml). Incubate for 2 to 4 h at 60 °C in a hot water bath" assertion.
- step reagentUsed _1 assertion.
- step reagentUsed _2 assertion.
- step labEquipmentUsed OBI_0400155 assertion.