Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAZQSfTf7hdqYE1qFLykuQB4o3Oftn9vZTlXwsJpPLiCM#assertion>. }
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- step type Step assertion.
- _1 label "cell suspension" assertion.
- _3 label "24 well culture plate" assertion.
- _2 label "cell suspension" assertion.
- step description "Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates." assertion.
- step labEquipmentUsed _3 assertion.
- step hasExperimentalOutput _1 assertion.
- step bioSampleUsed _2 assertion.