Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAVTi3POORCzo9w4YYq6YJfWLmMdcF-9g14yRqmTHbwu0#assertion>. }
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- step type Step assertion.
- OBI_0000136 label "incubator" assertion.
- BAO_0010014 label "falcon tube" assertion.
- OBI_0400155 label "water bath" assertion.
- step description "The falcon tube was kept into the 65°C incubator or water bath and mix gently by inversion after every 10 min till 45 min. After incubation, place the tube at room temperature for five min to reach to room temperature environment. Centrifuge the 50ml falcon tube for 5 min at 3000×g on room temperature. For next generation sequencing, the greater the genome size, lower is speed of initial centrifugation." assertion.
- step labEquipmentUsed BAO_0010014 assertion.
- step labEquipmentUsed OBI_0400155 assertion.
- step labEquipmentUsed OBI_0000136 assertion.