Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RACw4Ap2j_JyboYw-CXHknrMf8AF5mF6L5-mEYDJFI_L4#assertion>. }
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- step type Step assertion.
- _1 label "leaf tissues" assertion.
- _2 label "leaf tissues" assertion.
- _4 label "extraction buffer " assertion.
- _3 label " 50 mL Falcon tube" assertion.
- step description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin." assertion.
- step reagentUsed _4 assertion.
- step labEquipmentUsed _3 assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalInput _1 assertion.