Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion>. }
Showing items 1 to 6 of
6
with 100 items per page.
- step type Step assertion.
- _2 label "24 well culture plate" assertion.
- _1 label "cell suspension" assertion.
- step description "Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates." assertion.
- step labEquipmentUsed _2 assertion.
- step bioSampleUsed _1 assertion.