Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RA4i76gW52Hs0wwMZGJavBEJnPC-4huZHXTvu6zftDauI#assertion>. }
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- step type Step assertion.
- _1 label "DNA " assertion.
- C62218 label "70% ethanol" assertion.
- _3 label "TE" assertion.
- _4 label "tubes" assertion.
- UBERON_0036018 label "pellet" assertion.
- _2 label "DNA " assertion.
- step description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." assertion.
- step reagentUsed _4 assertion.
- step reagentUsed _3 assertion.
- step reagentUsed C62218 assertion.
- step hasExperimentalOutput _1 assertion.
- step bioSampleUsed _2 assertion.
- step bioSampleUsed UBERON_0036018 assertion.