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[Our results indicate that (1) single siRNAs complementary to ENaC subunits are sufficient to reduce ENaC transcripts, Na(+) channel activity, and fluid transport, but only silencing both the ? and ? ENaC subunits at the same time leads to an increase of ASF (from nearly 7 �m to more than 9 �m); (2) the ASF thickness obtained in this way is about half that measured after maximal CFTR stimulation in non-CF epithelia (10-14 �m); and (3) the pharmacological rescue of mutant CFTR increases the ASF to the same extent as ENaC silencing.]. Sentence from MEDLINE/PubMed, a database of the U.S. National Library of Medicine.
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