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[Most RA sera bound to recombinant amino-terminal region and domain I but not to domain L. This prompted us to use a recombinant polypeptide corresponding to the domain I of calpastatin as the antigen in a solid-phase ELISA to test sera from patients with various systemic rheumatic diseases and healthy controls.Anti-calpastatin domain I antibodies (ACAST-DI Ab), were detected by ELISA in RA, systemic lupus erythematosus (SLE), Sj�gren's syndrome and control sera at respective frequencies of 10, 9, 0 and 1%.]. Sentence from MEDLINE/PubMed, a database of the U.S. National Library of Medicine.
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